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KMID : 0350519960490020611
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Journal of Catholic Medical College
1996 Volume.49 No. 2 p.611 ~ p.619
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Increase of Endothelin-1 Synthesis induced by Sera from Severe Preeclamptic Women in the Cultured Human Umbilical Vein Endothelial Cells
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Abstract
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It is controversial whether sera from severe preeclamptic patients are diffusely and lethally toxic to human endothelial cells or act selectively on specific activation of their function. Endothelin-1 is a highly potent vasoconstrictor
synthesized
by
endothelial cells, which plays a role in hypertensive diseases.
In order to assess the effects of sera from preeclamptic patients on endothelial cell viability in vitro and endothelin-1 synthesis, the cultured human umbilical vein endothelial cells were incubated with media containing 10% sera form women with
either
preeclamptic patients or normal pregnancies for 24 hours or 48 hours. After then, their viability was measured by colorimetric MTT(3- (4, 5-dimethylthiazol-2yl)2, 5-diphenyl tetrazolium bromide) assay and their production of endothelin-1 was
measured.
We also measured the serum of endothelin-1 in sera obtained from the normal and severe preeclamptic pregnancies.
@ES The results are as follows:
@EN 1. The colorimetric MTT assay of cultured endothelial cells was performed by incubating for 24 hours or 48 hours with containing sera from women with either normal or sever preeclamptic pregnancies:
After 24 hours, the absorbances in the media treated with normal pregnancies and severe preeclampsia sera were 0.0718±0.0078 and 0.0837±0.0129, respectively and after 48 hours, they were 0.1133±0.0103 and 0.1268±0.0186, respectively. Serum
obtained
from severe preeclampsia did not affect endothelial cell viability.
2. The serum mean levels of endothelin-1 in normal and severe preeclamptic pregnancies were 22.66±8.60fmol/ml and 49.98±25.27 fmol/ml. The mean level in preeclamptic sera was significantly higher than that of normal pregnant sera (P<0.05).
3. After 24 hours, the mean amount of endothelic-1 stimulated by normal pregnant and severe preeclamptic sera were 37.52±18.41 fmol/ml nd 97.58±53.64 fmol/ml, respectively.
The mean amount of endothelin-1 in preeclamptic sera treated cells was significantly higher than that of normal pregnant sera (P<0.05).
The results suggest that the sera from severe preeclamptic women do not affect cell viability but act selectively on specific activation of their function such as endothelin-1 production.
And it is necessary that the identification and isolation of the putative serum factor (s) will be performed to resolve the pathogenesis in future.
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KEYWORD
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